12/29/2022 0 Comments Tetra enrich torrentMoreover, the influence of environmental factors (soil physical and chemical properties) on the nitrifying communities was evaluated. This study evaluates the diversity and abundance of nitrifying bacteria and archaea across different growth stages of maize using 16S amplicon sequencing. However, nitrifying bacteria and archaea inhabiting maize rhizosphere have not been fully explored. Nitrifying bacteria and archaea are ubiquitous and can transform ammonia locked up in soil or manure into nitrate, a more soluble form of nitrogen. We suggest that the presence of these unique genes and gene duplications may be associated with the environmental versatility of this group. Notably, genomes from the group I.1b have more gene duplications compared to the genomes from the group I.1a. These unique sequences code for proteins involved in control of DNA integrity, transporters, two-component systems and versatile CRISPR defense system. Detailed analysis of the genome revealed coding sequences that were completely absent from the group I.1a. evergladensis belongs to the group I.1b and shares only 40% of whole-genome homology with the closest sequenced relative Ca. Annotation of the reconstructed genome revealed many similarities of the basic metabolism with the rest of sequenced AOA. The de novo assembly of sequences resulted in one 2.95 Mb contig. The AOA enrichment was sequenced with the high throughput next generation sequencing platforms from Pacific Biosciences and Ion Torrent. Here, we describe the first complete genome of soil archaeon Candidatus Nitrososphaera evergladensis, which has been reconstructed from metagenomic sequencing of a highly enriched culture obtained from an agricultural soil. The lack of knowledge of metabolic pathways of soil AOA presents a critical gap in understanding their role in biogeochemical cycles. Many soils are dominated by AOA from the group I.1b, but the genomes of soil representatives of this group have not been sequenced and functionally characterized. To date, eight AOA genomes are available in the public databases, seven are from the group I.1a of the Thaumarchaeota and only one is from the group I.1b, isolated from hot springs. The activity of ammonia-oxidizing archaea (AOA) leads to the loss of nitrogen from soil, pollution of water sources and elevated emissions of greenhouse gas.
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